Effect of Culture Conditions on the Production of Growth Regulators and Extracellular Enzymes by Streptomyces Olivaceoviridis

It was proposed to find out the optimum environmental conditions and nutritional requirements for highest growth -regulating substance and enzyme production by Streptomyces olivaceoviridis. The optimum a-amylase and proteinase activity was at 45'C and 50'C, respectively. The test strain appeared to prefer pH 4.8 and 7.0 for the maximum productivity of a-amylase and proteinase, respectively. The highest potentiality for a-amylase and proteinase synthesis as well as the production of growth regulating substances were attained when S. olivaceoviridis was cultivated on media supplemented with starch as carbon source and peptone as nitrogen source. Furthermore, the addition of K2HP04 at 1.0 g/l (as phosphorus), zinc sulphate (as microelement) and yeast extract (as a vitamin source) to culture medium were found to enhance the production of growth regulating substances and enzymes by the experimental organism.